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Molecular Cytogenetic Analysis in Wheat

Dep. of Plant Pathology and the Wheat Genetics Resorce Center, Throckmorton Hall, Kansas State Univ., Manhattan, KS 66506-5502

^* Corresponding author.

Two technical innovations led to major advances in the molecular cytogenetic analyses of the wheat genome. The first innovation, the application of banding techniques, permitted distinction between heterochromatic and euchromatic regions and led to individual identification of metaphase chromosomes. The second innovation, the development of in situ hybridization protocols, permitted molecular analysis of chromosome structure, and physical localization of specific DNA sequences in individual chromosomes. The applications of banding and in situ hybridization techniques in cytogenetics, plant breeding, gene mapping, and in assaying alien chromatin introgression are briefly discussed. A connection is made to the pioneering contributions of E.R. Sears, without which, rapid progress in the molecular cytogenetic analysis of wheat would not have occurred.

Received for publication April 24, 1992.