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Batch Anion Exchange Separation and Quantification Of [¹⁴C]Hexose from [¹⁴C]Sucrose

Dep. of Soil and Crop Sci., Texas A&M Univ., College Station, TX 77843-2474
USDA-ARS, Tropical Agric. Res. Stn., Mayagüez, PR 00681-0070

^* Corresponding author.

Techniques quantifying ¹⁴C]photoassimilate partitioning between hexose and sucrose can identify developmental and regulatory changes in sugar metabolism of sorghum [Sorghum bicolor (L.) Moench]. The objective of this study was to develop a laboratory method for separating and quantifying [¹⁴C]hexose and [¹⁴C]sucrose in numerous plant organ extracts simultaneously. The described method combines easily with available assays for [¹⁴C]starch and [¹⁴C]structural components and for total fructose, glucose, sucrose, and starch. Treatment with hexokinase is followed by batch treatment with anion exchange resin. Hexose, as hexose phosphate, binds (>95%) to the resin, and sucrose remains unbound (>95%). Radiolabel in extracts is nearly completely recovered (99%). Determinations are reproducible (SE = 1.3%). This method was applied to identify differences in sugar metabolism between organs and among growth stages of field-grown sorghum.

Received for publication April 28, 1992.

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