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Use of Tassel-Seed (Ts-5) Maize for Assimilate Transport Studies Using Intact or Detached Tassel Branches

Dep.of Extension Horticulture, Univ. of Georgia, Athens, GA 30602
USDA-ARS, Natl. Ctr. for Agric. Utilization Res., Seed Biosynthesis Res. Unit, 1815 N. University St., Peoria, IL 61604
Dep.of Horticulture, Pennsylvania State Univ., University Park, PA 16802

^* Corresponding author.

The anatomy of normal maize (Zea mays L.) ears precludes easy access to the kernel pedicel tissue for in vivo assimilate transport experiments. We investigated the possibility of using the Tassel-seed (Ts-5) mutant of maize as a model system for studies of assimilate movement into developing maize kernels. Hypodermic needles (26 gauge, 9.5 mm long) inserted under the pedicel epidermis of intact plants were used to introduce solutes into the pedicel free space. Additionally, excised tassel branches were incubated in buffered solutions containing 100 mM ¹⁴C.labeled sugars. The movement of apoplastic and symplastic fluorescent dyes as well as ¹⁴C-iabeled assimilates and incubation solutions showed that Ts-5 maize provides a useful experimental system for study of the mechanism of assimilate transport into developing kernels.

Received for publication April 23, 1992.

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