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Published in Crop Sci 32:1502-1505 (1992)
© 1992 Crop Science Society of America
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Nulls for the Major Soybean Bowman-Birk Protease Inhibitor in the Genus Glycine

Juliann M. Domagalski, Krishna P. Kollipara, Anne H. Bates, David L. Brandon, Mendel Friedman and Theodore Hymowitz*

Dep. of Agronomy, 1102 S. Goodwin Ave., Univ. of Illinois, Urbana, IL 61801
Dep. of Agronomy, 1102 S. Goodwin Ave., Univ. of Illinois, Urbana, IL 61801
USDA-ARS, Western Regional Res. Ctr., 800 Buchanan St., Albany CA 94710

* Corresponding author.

The Bowman-Bilk protease inhibitor (BBI) is a major antinutritional factor in soybean [Glycine max (L.) Merr.]. This study was conducted to determine nulls for the major soybean BBI in the genus Glycine. A monoclonal antibody-based competitive enzyme-linked immunosorbent assay (ELISA) was used to rapidly detect the relative levels of the major soybean BBI in unpurified seed extracts. A total of 12 690 accessions from 13 species were tested. All of the G. max and G. soja Siebold & Zucc. accessions from the USDA Soybean Germplasm Collection contained BBI. The BBI nulls were found hi several of the wild perennial species: G. curvata Tind., G. cyrtoloba Tind., G. latifolia (Benth.) Newell & Hymowitz, G. microphylla (Benth.) Tind, G. tabacina (Labill.) Benth., and G. tomentella Hayata. The absence of BBI was confirmed by additional immunochemical methods and by enzymatic determination of trypsin and chymotrypsin inhibition. The BBI nulls identified in this study could potentially be utilized in the production of a soybean cultivar lacking the major soybean BBI.

Research supported in part by the Illinois Agric. Exp. Stn., USDA Res. Agreement no. 58-91H2-M-239, and a grant to T. Hymowitz from the Illinois Soybean Program Operating Board (88-14-87-3).

Received for publication November 12, 1991.




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Copyright © 1992 by the Crop Science Society of America.