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Published in Crop Sci 32:362-366 (1992)
© 1992 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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Cytological Comparison of Amplified Chromosome Segments in Four Tobacco Doubled Haploids

Sandra M. Reed*, Joyce A. Burns and E. A. Wernsman

USDA-ARS Crops Res. Lab., Oxford, NC 27565-1168
Dep. of Crop Science, North Carolina State Univ., Raleigh, NC 27695-7620

* Corresponding author.

Atypical quadrivalents, which appear to be the result of pairing between amplified homologous segments in nonhomologous chromosomes, have been found in tobacco (Nicotiana tabacum L.) doubled haploid (DH) x cultivar hybrids. The purpose of this study was to determine if the same amplified chromosomes are involved in these quadrivalents in different DH lines. Chromosome pairing was studied in hybrids between four DHs, of which two were derived from ‘NC95’ and the other two from ‘C139’. Results of this study indicate that all four of the DHs contain two homologous amplified chromosomes regions. The chromosomal locations of both of these amplified segments in three of the DHs appear to be the same. One of the amplified segments in the fourth DH is located on the same and the other on a different chromosome than in the other three DHs. The difference in location of one of the amplified segments in this fourth DH could be the result of a translocation or the amplification of nontandem multigene families. That similar genetic sequences have been amplified in all four DHs studied suggests there may be some specificity to the DNA amplification that occurs as a result of anther culture in tobacco.

Joint contribution of the USDA-ARS and the North Carolina Agric. Res. Serv.

Received for publication June 19, 1991.




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