HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Wofford, D. S. Articles by Baltensperger, D. D. Search for Related Content PubMed Articles by Wofford, D. S. Articles by Baltensperger, D. D. Agricola Articles by Wofford, D. S. Articles by Baltensperger, D. D.

Tissue Culture Regeneration of Desmodium

Dep. of Agronomy, Univ. of Florida, 2183 McCarty Hall, Gainesville, FL 32611

D. D. Baltensperger

Univ. of Nebraska, Panhandle Res. and Ext. Ctr., Scottsbluff, NE d 69361

^* Corresponding author.

Efficient tissue culture techniques for plant regeneration are known for many crops, but very considerably among species. These procedures need to be investigated, however, in crops that have no known method of regeneration. Six genotypes of two species of Desmodium were evaluated for tissue culture response on two protocols, an L2 basal salts + growth regulators embryogenic protocol and an MS basal salts + growth regulators organogenic protocol. Significantly greater callus growth was observed on the L2 callus induction medium for four of the six genotypes, with an average of 476.6 mg callus^–1 for all genotypes on L2 compared with 47.9 mg callus^–1 for the MS treatment. Based on L2 callus fresh weight data, genotypes could be classified into two groups, good and poor callus producers. Despite the overall poor callus growth on MS, shoot buds were induced for five of the six genotypes on the MS shoot induction medium. No genotypes produced somatic embryos on the L2 protocol; however, two genotypes, CIAT 6123 and CIAT 13083, did exhibit organogenesis on the L2 protocol. Plants were regenerated from CIAT 6123 on the L2 system. No shoot development was observed for any genotype on the MS treatment. Regenerated plants were phenotypically normal and no somaclonal variation was observed. Media modifications will be necessary in order to successfully manipulate Desmodium genotypes in vitro.

Received for publication April 1, 1991.