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In Vitro Culture Responses of Alyceclover Genotypes on Four Media Systems

Dep. of Agronomy, Univ. of Florida, 2183 McCarty Hall, Gainesville, FL 32611
Univ. of Nebraska, Panhandle Res. and Ext. Ctr., Scottsbluff, NE 69361
Dep. of Agronomy, Univ. of Florida, 2183 McCarty Hall, Gainesville, FL 32611

^* Corresponding author.

Tissue culture protocols for regeneration are widely variable among plant species. Without known techniques for in vitro regeneration of a given species, many molecular methods cannot be applied in crop improvement programs. In this study, the in vitro response of six genotypes of alyceclover, Alysicarpus vaginalis (L.) DC., was evaluated on two organogenic systems, B5 and MS basal salts plus growth regulators, and two embryogenic systems, L2 and SH basal salts plus growth regulators. Callus growth of genotypes on the L2-based medium was significantly greater than on the other treatments, with significantly less growth on the MS callus induction medium. Shoot bud induction, but not embryo induction, was observed for all six genotypes on one or more media treatments. Genotype FL-1 initiated shoot buds on three of the four media treatments, B5, L2, and MS. Genotype FL-10 produced the highest percent of call! with induced shoot buds, with 63 and 89% for the B5 and MS treatments, respectively. Plants of four genotypes were regenerated after transfer to root induction or embryo germination medium. Genotype FL-10 produced 128 and 19 plants when cultured on the L2 and B5 systems, respectively. Genotypes FL-1, FL-4, and FL-100 produced a total of 24 plants when cultured on the MS system. All regenerated plants were phcnotypically normal in appearance.

Received for publication March 4, 1991.