HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Tahara, M. Articles by Johnson, R. C. Search for Related Content PubMed Articles by Tahara, M. Articles by Johnson, R. C. Agricola Articles by Tahara, M. Articles by Johnson, R. C.

Quantification of Abscisic Acid in Wheat Leaf Tissue by Direct Enzyme Immunoassay

Nippon Steel Inc., Biotechnology Div., 2-6-3 Otemachi, Chiyoda-ku, Tokyo, Japan
Dep. of Agronomy, Oklahoma State Univ., Stillwater, OK 74078
USDA-ARS, Forage and Range Res. Lab., Logan, UT 84322
USDA-ARS, 59 Johnson Hall, Washington State Univ., Pullman, WA 99164

^* Corresponding author.

The plant growth hormone abscisic acid (ABA) modulates plant responses and adaptations to environmental stress. The objective of this study was to develop a procedure which would facilitate broad-scale screening of wheat (Triticum aestivum L. em. Thell.) germplasm in which ABA content or rate of accumulation in leaf tissue is the selection criterion. The effects of extraction time, C18 reverse-phase chromatography, solvent volatilization, and dilution of sample extract on quantification of ABA by direct enzyme immunoassay (EIA) were investigated. Results showed that complete volatilization of extraction solvent did not affect ABA determination. Extraction of ABA from wheat leaves, using 800 mL L^–1 methanol (MeOH), was complete after 24 h. Interference to EIA due to MeOH was eliminated when MeOH was diluted to 80 mL L^–1. No apparent inhibitors to EIA were detected by a parallelism test of dilution curves or by partial purification of leaf extract using C18 reverse-phase chromatography. Simplified and effective procedures are proposed which facilitate the preparation of a large number of leaf samples at minimal expense and labor without sacrificing accuracy of ABA estimation.

Received for publication May 29, 1990.