Crop Science Journal of Natural Resources and Life Sciences Education
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Published in Crop Sci 31:893-899 (1991)
© 1991 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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Retriction Fragment Length Polymorphisms Can Differentiate among U.S. Maize Hybrids

J. S. C. Smith* and O. S. Smith

Dep. of Biotechnology
Dep. of Data Management, Pioneer Hi-Bred International, Inc. Plant Breeding Division, 7250 NW 62nd Avenue, Johnston, IA 50131.

* Corresponding author.

Previous methods to differentiate U.S. maize hybrids using biochemical data have been criticized on the basis that the genome has been inadequately sampled. The objective of this study was to utilize an additional set of highly polymorphic genetic markers (restriction fragment length polymorphisms; RFLPs) to establish the degree to which these data discriminate among 78 elite maize (Zea mays L.) U.S. hybrids selected with a bias toward including hybrid pairs previously undifferentiated by biochemical data. Thirty-eight probes revealed 288 RFLP variants across all hybrids. RFLP and biochemical data clustered most hybrids into similar groupings. For those hybrids of known pedigree, distances between hybrids on the basis of pedigree showed a correlation of r = 0.91 with RFLP distances. Where available, field performance data gave identical groupings of hybrids as were shown by RFLP data. These data show that RFLPs can provide a practical means of differentiating hybrids.

Received for publication May 22, 1990.


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