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Starch Gel Electrophoresis Used for Identification of Turf-type Cynodon Genotypes

Dep. of Soil & Crop Sciences, Texas A&M Univ., College Station, TX 77843

*Corresponding author.

A reliable technique is needed to facilitate the identification of cultivars of turf-type Cynodon species because of difficulties encountered using morphological traits alone. This study was conducted to determine the potential for using starch gel electrophoresis to identify 21 Cynodon genotypes. Six enzyme systems (aconitase, alcohol dehydrogenase, phosphoglucomutase, phosphoglucoisomerase, shikimic acid dehydrogenase, and triosephosphate isomerase) were used because of ease of resolution. Plant material was grown in a greenhouse or growth chamber and crude protein extracts were made from recently emerged leaf blades. Examination of banding patterns allowed identification of 16 unique genotypes (‘A-22’, ‘A-29’, ‘Bayshore’, ‘Everglades’, ‘FB-119’, ‘Midiron’, ‘Midway’, ‘Ormond’, ‘Santa Ana’, ‘Sunturf’, ‘Tiffine’, ‘Tifgreen II’, ‘Tiflawn’, ‘Tifway’, ‘Tifway II’, and ‘Vamont’) using three of the enzyme systems (aconitase, phosphoglucomutase, and phosphoglucoisomerase). The five remaining genotypes were separated into two distinct groups: (i) ‘Pee Dee’, ‘Tifgreen’, and ‘Tifdwarf’ and (ii) ‘Texturf 10’ and ‘Texturf if’. It was concluded from this study that starch gel electrophoresis is useful in genotypic identification with Cynodon species. Additional isoenzyme characterizations are required to achieve individual identification of all genotypes in the collection.

Received for publication November 20, 1989.