HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Parrot, W. A. Articles by Bouton, J. H. Search for Related Content PubMed Articles by Parrot, W. A. Articles by Bouton, J. H. Agricola Articles by Parrot, W. A. Articles by Bouton, J. H.

Aluminum Tolerance in Alfalfa as Expressed in Tissue Culture

Dep. of Agronomy, Univ. of Georgia, Athens, GA 30602

^* Corresponding author.

Acid soils whose pH is low enough for A1 to become soluble are found throughout the world and greatly limit growth of Al-sensitive crops like alfalfa, Medicago sativa L. Alfalfa germplasms were selected to overcome these conditions and are now available for testing. The objective of this study was to test performance of an acid-tolerant germplasm (AT), and an acid-sensitive check germplasm (AS), in acid, Al-toxic cell culture conditions. Both germplasms were tested for growth as callus in normal Biaydes medium, a Blaydes medium modified by lowering pH and levels of Ca and P, and on the same modified medium but with a toxic Al level. The modified Blaydes medium depressed callas growth of both germplasms relative to their growth on normal Blaydes. This depression was equal for both germplasms of the first 6 wk of culture, but by the 8th wk, the AT germplasm outperformed the AS germplasm. The addition of Al further decreased growth of AS callus but not AT callus. This suggests that plants from the AT germplasm rapidly express Al tolerance at the tissue level. With increasing time in culture, the AT germplasm also expresses acid tolerance in culture. The differences in callus growth shown by genotypes within germplasms indicates the cell culture procedure can be used to screen genotypes for acid or Al tolerance. Significant genotypic variability for acid, Al tolerance is still present within the germplasm, suggesting that continued progress from selection should still be possible.

Received for publication May 25, 1989.

This article has been cited by other articles:

				B. Narasimhamoorthy, E. B. Blancaflor, J. H. Bouton, M. E. Payton, and M. K. Sledge A Comparison of Hydroponics, Soil, and Root Staining Methods for Evaluation of Aluminum Tolerance in Medicago truncatula (Barrel Medic) Germplasm Crop Sci., February 6, 2007; 47(1): 321 - 328. [Abstract] [Full Text] [PDF]

				M. K. Sledge, P. Pechter, and M. E. Payton Aluminum Tolerance in Medicago truncatula Germplasm Crop Sci., August 26, 2005; 45(5): 2001 - 2004. [Abstract] [Full Text] [PDF]

				M. K. Sledge, J. H. Bouton, M. Dall'Agnoll, W. A. Parrott, and G. Kochert Identification and Confirmation of Aluminum Tolerance QTL in Diploid Medicago sativa subsp. coerulea Crop Sci., July 1, 2002; 42(4): 1121 - 1128. [Abstract] [Full Text] [PDF]