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Association of Major Seed Components with a Shriveled-Seed Trait in Soybean

J. W. Burton

R. G. Palmer and R. C. Shoemaker

Dep. of Genetics, 8 Curtiss Hall, Iowa State Univ., Ames, IA 50011
Dep. of Crop Science and USDA-ARS, Plant Science Bldg., 3127 Ligon St., North Carolina State Univ., Raleigh, NC 27695

^* Corresponding author.

Soybean meal accounts for 70 to 75% of the international trade in high-protein plant products. Soybean protein is nutritionally deficient in certain amino adds. This deficiency has been associated with major storage protein subunits. The study of mutants affecting major seed components may prove useful in efforts to improve the nutritional value of the soybean [Glycine max (L.) Merr.i. Studies of the association of a shriveled-seed mutant in soybean with major seed components are presented. Sodium dodecyl sulfate-polyacrylamide electrophoresis (SDS-PAGE) analysis of severely shriveled seed of the mutant, 328-1, showed a reduced level of a polypeptide of 48 kilodalton (Kd) molecular weight subsequently identified the ß-subunit of the 7S storage protein. Densitometer scans of SDS-PAGE protein gels from normal and shriveled seed confirmed changes in the levels of other proteins. The detectable accumulation of the ß-subunit during seed development occurred later relative to the and -subunits in the mutant genotype than in its parents. Differences in oil content in the mutant and parental genotypes are indicated by nuclear magnetic resonance (NMR) analysis. These differences are due to a genotype x phenotype interaction. Also, it has been shown that genotype effects on total protein content are not significant (P > 0.05).

Received for publication April 29, 1988.

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