HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Smith, A. J. Articles by Seif, R. D. Search for Related Content PubMed Articles by Smith, A. J. Articles by Seif, R. D. Agricola Articles by Smith, A. J. Articles by Seif, R. D.

Phosphoenolpyruvate Carboxylase and Pyruvate Kinase Involvement in Protein and Oil Biosynthesis during Soybean Seed Development

Univ. of Illinois
USDA-ARS and Dep. of Agronomy, Univ. of Illinois
Dep. of Agronomy, Univ. of Illinois, 1102 S. Goodwin, Urbana, IL 61801

^* Corresponding author.

During soybean [Glycine max (L.) Merr.] seed development, sucrose translocated to the seed is metabolized to precursors for protein and oil, the major components of mature soybean seed. while breeders have had success in selecting for increased seed protein or oil concentration, there is little understanding the metabolic events that regulate the partitioning of carbon into these seed components. Enzyme reactions that may determine carbon partitioning to seed protein and oil were examined in six soybean genotypes exhibiting genotypic differences in concentration of seed protein and oil. Developing seed were assayed for the enzymes phosphoenolpyruvate (PEP) carboxylase, pyruvate kinase, malate dehydrogenase, NADP-malic enzyme, and aspartate and alanine aminotransferases. Correlations between these data and the protein and oil concentrations of each sample were determined. There was no significant difference between the correlation of pyruvate kinase activity with the oil and that with the protein concentration of the seed over development. The correlation of PEP carboxylase activity with protein was generally higher than with oil, suggesting that much of the oxaloacetate (OAA) produced may be used for synthesis of protein precursors. However, the high r values between PEP carboxylase and oil suggested that OAA may also be converted to malate and then to pyruvate (a precursor for oil) via a transhydrogenase system. Therefore, PEP carboxylase and pyruvate kinase activities appeared to contribute to a complex interaction that regulates the metabolic flow of glycolytic carbon into precursors for both protein and oil biosynthesis during soybean seed development.

Received for publication April 25, 1988.

This article has been cited by other articles:

				J. Schwender, Y. Shachar-Hill, and J. B. Ohlrogge Mitochondrial Metabolism in Developing Embryos of Brassica napus J. Biol. Chem., November 10, 2006; 281(45): 34040 - 34047. [Abstract] [Full Text] [PDF]

				C. Hernandez-Sebastia, F. Marsolais, C. Saravitz, D. Israel, R. E. Dewey, and S. C. Huber Free amino acid profiles suggest a possible role for asparagine in the control of storage-product accumulation in developing seeds of low- and high-protein soybean lines J. Exp. Bot., July 1, 2005; 56(417): 1951 - 1963. [Abstract] [Full Text] [PDF]

				H. Rolletschek, F. Hosein, M. Miranda, U. Heim, K.-P. Gotz, A. Schlereth, L. Borisjuk, I. Saalbach, U. Wobus, and H. Weber Ectopic Expression of an Amino Acid Transporter (VfAAP1) in Seeds of Vicia narbonensis and Pea Increases Storage Proteins Plant Physiology, April 1, 2005; 137(4): 1236 - 1249. [Abstract] [Full Text] [PDF]