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Polyploids from 2x-4x and 4x-2x Crosses in Red Clover

Dep. of Agronomy, Univ. of Kentucky, Agric. Sci. Bldg., North, Lexington, KY 40546

^* Corresponding author.

Efficient methods of producing tetraploids are important to red clover (Trifolium pratense L.) breeders. An effort to widen the genetic base of tetraploid experimental lines via gametic nonreduction provided an opportunity to further examine this methodology. The objectives of this research were to further evaluate the frequency of triploid and tetraploid progeny from 2x-4x and 4x-2x crosses in red clover, and to examine the use of seed dockage sieves as a labor saving device in separating polyploid from diploid seeds. A few triploids were found among progenies from 2x-4x crosses in contrast to our earlier research, but the frequency was considerably lower than from 4x-2x crosses. Tetraploids were more frequent among progeny of the 4x-2x cross than progenies from 2x-4x crosses. Isolation of polyploid seeds was facilitated by sieving seeds and growing only those from the larger sieve sizes. The most efficient method of producing both triploids and tetraploids was by 4x-2x crosses rather than by 2x-4x crosses. However, a leaf mark or other gene marker is necessary to eliminate tetraploid selfs.

Received for publication March 17, 1988.