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Prposed Schemes for Mass-Extraction of Doubled Haploids of Cotton

Dep. of Soil & Crop Sciences, Texas A&M Univ., College Station, TX, 7783-2474
Dep. Crop Science, North Carolina State Univ., Raleigh, NC, 27695-7620

^* Corresponding author.

We propose (i) synthesis of hybrid-eliminating, haploid-inducing (HEHP) lines and populations homozygous for the Le₂ ^day hybrid lethality allele and the Se allele for semigamous reproduction, (ii) cross pollination of HEHP plants with pollen of normal cotton (Gossypium hirsutum and G. barbadense) to form inviable true hybrids, but viable haploids, and (iii) bulk treatment of all seed or young viable seedlings with colchicine to recover doubled haploids en masse. Doubled paternal haploid plants and sectors will be phenotypically unique, as distinguished by fertility, size of plant parts, and expression of genetic marker(s). Inclusion of male sterility in segregating HEHP populations will facilitate manual or natural cross pollination, allowing large-scale usage. To convert cytoplasms of HEHP materials, available semigamous lines with the desired cytoplasm can be crossed with a HEHP pollen parent to obtain paternal haploid progeny that can be colchicine-doubled. The HEHP materials should facilitate all types of cytogenetic manipulations involving extraction of doubled (paternal) haploids. The basic concepts and methods proposed might be extrapolatable to other crops.

Received for publication April 28, 1987.

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