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Published in Crop Sci 28:1010-1012 (1988)
© 1988 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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Inheritance of Double Nulls for Protein Components of Soybean Seed

J. A. Prischmann and T. Hymowitz*

Dep. of Agronomy, 1102 S. Goodwin Ave., Urbana, IL 61801

* Corresponding author.

The objective of this study was to determine whether it was possible to develop in soybean [G. max (L.) Merr.] seeds double null genotypes for the Kunitz trypsin inhibitor (ti) null in reciprocal combinations with genotypes lacking lectin (le), lipoxygenase-1 (/xl), µ-amylase (sp1), and urease (Eul-sun). Homozygous double recessive soybean lines were obtained for all eight combinations. From F2 data collected using colorimetric, immunological, and electrophoretic tests, we demonstrated that the Kunitz trypsin inhibitor locus segregated independently of the iectin, lipoxygenase-1, urease and {alpha}-amylase loci.


Research, partly supported by the Standard Oil-Univ. of Illinois Center of Excellence in Crop Molecular Genetics and Genetic Engineering, was from thesis by senior author in partial fulfillment of M.S. degree requirements at the Univ. of Illinois.

Received for publication August 19, 1987.





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