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Published in Crop Sci 27:519-521 (1987)
© 1987 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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Assying for Linkage between Rps and Isozyme Loci in Soybean1

Brian D. Rennie, Wallace D. Beversdorf and Richard I. Buzzell2

Linkage assays were run with soybean [Glycine max (L.) Merr.] lines differing for Rps and isozyme alleles, in an attempt to identify markers for Rps loci. The F2 seedlings were screened for resistance to specific races of Phytophthora megasperma (Drechs.) f.sp. glycinea (Hildeb.) Kuan & Erwin and cotyledon samples were assayed for isozyme patterns by means of starch gel electrophoresis. Alleles at the Rps1, Rps3, Rps4, Rps5, Rps6,, and Rps? loci were assayed for linkage with isozyme alleles at the Aco4, Dial, Idhl, IdH2, and Pgm1 loci. No linkages were detected for the 18 Rps-isoxyme pairs tested.

Key Words: Electrophoresis • Mapping • Glycine max (L.) Merr. • Phytophthora megasperma (Drechs.) • Root rot resistance

1 Contribution from the Crop Science Dep., Univ. of Guelph, and Agric. Canada. To be included in a dissertation by the senior author in partial fulfillment of a Ph.D, degree. This research was supported in part by Agric. Canada and the Ontario SoyaBean Growers' Marketing Board.

2 Graduate research assistant and associate professor, Crop Science Dep., Univ. of Guelph, Guelph, Ontario NIG 2W1, and soybean breeder, Agric. Canada Res. Stn., Harrow, Ontario NOR 1GO.

Received for publication July 21, 1986.




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