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Improvement in Callus Growth and Plantlet Formation in Creeping Bentgrass¹

Optimizing callus growth and plantlet formation from somatic callus is advantageous in the recovery of desirable variants using in vitro cell selection methods. Using 24-week-old ‘Penncross’ creeping bentgrass (‘Agrostis palustris Huds‘.) stock callus (SC), callus growth and plantlet formation were evaluated from plated SC previously cultured in suspension for 12 and 24 days and SC not previously cultured in suspension. In addition, callus growth and plantlet formation were evaluated from SC plated at aggregate sizes of 0.5- to 1.0-mm and 0.25- to 0.5-mm diam and aggregate densities of 1, 3, and 5 mg.50 mm^–2. Callus growth was twofold greater from SC previously cultured in suspension than from SC not previously cultured in suspension. Plantlet formation was inhibited from SC previously cultured in suspension (<10 plantlets/dish) compared to SC not previously cultured in suspension (250 to 450 plantlets/dish). Plated SC aggregates of 0.5- to 1.0-mm diam generally had greater growth than aggregates 0.25-to 0.5-mm diam. At both aggregate size dimensions, greatest growth occurred from SC plated at 1 mg.50 mm^–2 plating density. Aggregates 0.5- to 1.0-mm and 0.25- to 0.5- mm diam had the greatest plantlet formation at 1 mg.50 mm^–2 and 1 and 3 mg.50 mm^–2 densities, respectively.

Key Words: Aggregate size • Plating density • Cell suspension culture • Solid medium • Agrostis palustris Huds

² Research assistant, professor of agronomy, and professor of weed science, Mississippi State Univ., Mississippi State, MS 39762.

Received for publication February 28, 1986.