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Alfalfa Triploids with Functional Male and Female Fertility¹

Alfalfa (Medicago saliva L.) triploids (2n=3x = 24) were produced by crossing tetraploid cultivated alfalfa with cultivated alfalfa at the diploid level (CADL) derived by haploidy. Alfalfa triploids usually function only as seed parents in crosses; however, eight of nine triploids in this study functioned as the pollen parent in 3x-3x, 4x-3x, or 6x-3x crosses. Moreover, two triploids produced self-progeny. In 3x-3x crosses, most progeny had 31 or 32 chromosomes, but progeny with 29,35,45,46,47,48, and 58 chromosomes were also produced. Triploid self-progeny had 31, 32, 36, 45, 46, 47, 48, and 52 chromosomes. Several hexaploids (2n=48) and near hexaploids (2n=45-52) were produced by sexual polyploidy in 3x self, 3x-3x, 3x-6x, and 6x-3x matings.

Key Words: Medicago sativa L. • Gamete • Diploid • Tetraploid • Hexaploid • Octoploid

² Specialist (present address: Dep. of Microbiology, Montana State Univ., Bozeman, MT 59715) and professor, respectively, Dep. of Agronomy, Univ. of Wisconsin, Madison, WI 53706.

Received for publication May 13, 1985.