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Sequential Fiber Analysis of Forage as Influenced by Sample Weight¹

Situations exist where sample weight may be limiting to sequential analysis of forage fiber. The minimum sample weight that can be used to obtain reliable values for sequential fiber analysis constituents is not known. Our objective was to determine the effect of sample weight on sequential forage fiber digestion values. Sample weights of 0.05, 0.10, 0.20, 0.30, 0.40, 0.50, 1.00, and 2.00 g were evaluated for each of three species, alfalfa (Medicago sativa L.) sorghum [Sorghum bicolor (L.) Moench.], and reed canarygrass (Phalaris arundinacea L.). Five samples were measured for each sample-weight, species combination. Sequential forage fiber analysis for neutral detergent fiber, acid detergent fiber, hemicellulose, cellulose, lignin, and insoluble ash was accomplished using glass microfiber filters and sintered glass crucibles. Although means were not greatly influenced by sample weight, variances were increased by reduced sample weight. Sample weights ranging from 0.30 to 2.0 g resulted in reasonably precise and accurate estimates of fiber constituent values.

Key Words: Alfalfa • Sorghum • Reed canarygrass • Forage quality • Hemicellulose • Cellulose • Lignin

² Assistant professors, and professor, Dep. of Agronomy, Purdue Univ., West Lafayette, IN 47907.

Received for publication September 14, 1984.