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Published in Crop Sci 25:121-123 (1985)
© 1985 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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Inoculation of Seedling Cuttings for Screening the Soybean Against Races of Phytophthora megasperma f. sp. glycinea1

Mung V. Nguyen, Marvin W. Rode and Donald W. Payne2

Young soybean [Glycine max (L.) Merr.] seedlings grown sand were cut at the base of their hypocotyls, and mycelia of Phytophthora megasperma Drechs. f. sp. glycinea Kuan and Ervin (Pmg) were smeared onto the cut surfaces. The cuttings were then transferred to water. The cuttings were classified as "resistant" if they continued to grow and had produced roots on the hypocotyls 7 days after inoculation; they were classified as "susceptible" if the hypocotyls decayed and collapsed with no root initiation. Similar results were obtained with cuttings vs. hypocotyl in oculations when soybean cultivars differing in their reactions to races 1 and 5 were tested. The method described herein is compared to several other currently used techniques, and their respective advantages and disadvantages are discussed. In general, inoculating soybean seedling cuttings for Phytophthora screening is efficient, reliable, and inexpensive. Compared to other methods, it is simple and requires very little equipment and working space in the laboratory, but requires more inoculum.

Key Words: Glycine max (L.) Merr. • Disease screening • Hypocotyl inoculation

1 Contribution from Illinois Foundation Seeds, Inc., P.O. Box 722, Champaign, IL 61820.

2 Soybean breeder, former research director (present address: Parke Seeds Inc. Marshall, IN 47859), and research technician, respectively.

Received for publication May 23, 1983.




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