HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Altman, D. W. Articles by Gengenbach, B. G. Search for Related Content PubMed Articles by Altman, D. W. Articles by Gengenbach, B. G. Agricola Articles by Altman, D. W. Articles by Gengenbach, B. G.

Confirmation by Mitochondrial DNA Analysis of a Euplasmic Spring Wheat Expressing a Dominant Male-Sterility Gene¹

A dominant male-sterility gene has had limited utilization in wheat (Triticum aestivum L.) breeding partly because appropriate lines have alien (T. tauschii Schmal.) cytoplasm. The dominant allele for male-sterility was though to have been transferred into a euplasmic (T. aestivum cytoplasm) background, but the cytoplasmic constitution of this line had not been confirmed. Electrophoretic gel patterns of mitochondrial DNA (mtDNA) a putative euplasmic line which segregates for the dominant male-sterility allele were compared with gel patterns of mtDNA of known T. aestivum and T. tauschii cytoplasm lines following digestion with the restriction endonucleases PstI, EcoRl, BamHI, Sall, Xhol, and Hindlll. All restriction patterns of the putative euplasmic line verified that the cytoplasm was from T. aestivum. Euplasmic sources of dominant male-sterility should have greater value than alloplasmic lines for future genetic and breeding studies using this gene.

Key Words: Triticum aestivum L. • Triticum tauschii Schmal • Restriction endonucleases • Cytoplasm • Electrophoresis

² Research geneticist, USDA-ARS, Cotton and Grain Crops Genetic Research Unit, College Station, TX 77841 (former research specialist. Dep. of Agronomy and Plant Genetics, Univ. of Minnesota), junior scientist, and professor, Dep. of Agronomy and Plant Genetics, Univ. of Minnesota, St. Paul, MN 55108.

Received for publication October 6, 1983.