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Artificial inoculation of sorghum [Sorghum bicolor (L.) Moench] with maize dwarf mosaic virus (MDMV) has led to the release of tolerant inbred lines and hybrids. When a spray gun is used for inoculation, differences in pressure and inoculum concentrations used during inoculatlon could result in a selectively affected disease rating among genotypes. Ten inbred line sorghum genotypes of known MDMV reaction were artificially inoculated at 1:10 and 1:100 (w/v), tissue to buffer concentration at 0, 1.4, 4.2 and 7.3 kg/cm2 pressure and rated on an individual plant basis 21 days after inoculation. Pressure, concentration, and genotype Disease Severity Index (DSI) were varied. Significant interactions were detected between pressure and concentration of inoculum. High pressures increased genotypic susceptibility as did greater virus concentration. In this study, even though DSI varied under different pressure and concentration combinations, genotypes responded uniformly and rankings remained unchanged.
Key Words: Grain sorghum plant virus Cultivar response
2 Professor of plant sciences, and associate professor of soil & crop sciences, Texas Agrie. Exp. Stn., respectively
Received for publication January 8, 1982.
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