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Long-Term Pollen Storage of Pearl Millet¹

The purpose of this research was to develop a practical method for long-term pollen storage of pearl millet (Pennisetum americanum L. Leeke). Pearl millet pollen with 7.5% moisture stored for 185 days in plastic zip-lock bags at –73 C gave 100% seed set when used to pollinate cytoplasmic male sterile (cms) ‘Tift 23DA’ heads. Pollen dried to 2.2% also gave 100% seed set on cms heads after 129 days of storage. Frozen pollen with 6 to 7% moisture content that was thawed for 3 hours, refrozen, and later used in crosses produced 100% seed set.

Key Words: Freezing pollen • Pollen moisture content

² Research geneticist, research plant pathologist, research geneticist, and research agronomist, ARS-USDA and the Univ. of Georgia, College of Agricultural Experiment Stations, Coastal Plain Station, Tifton, GA 31793.

Received for publication May 3, 1982.