Crop Science Journal of Natural Resources and Life Sciences Education
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Published in Crop Sci 23:167-168 (1983)
© 1983 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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Determination of Protein Percentage of Wheat Vegetation at Two Growth Stages by near Infrared Reflectance1

C. M. LÖffler and R. H. Busch2

The potential usefulness of near infrared reflectance analysis to study genetic variability for plant protein percentage in spring wheat (Triticum aestivum L.) was evaluated using a Technicon Near Infrared Analyzer (Infra Alyzer 300). Kjeldahl protein values were used calibrate the instrument for plant samples taken at anthesis and maturity. Reflectance readings, obtained using five filter positions, were fitted to Kjeldahl values using multiple linear regression, and regression constants were obtained. Coefficients of determination (R2) were 0.75 and 0.89 for samples at anthesis and maturity, respectively. Correlation coefficients between Kjeldahl and near infrared reflectance analyses were calculated from four sets of independent samples. The correlation coefficients for samples at anthesis and maturity were 0.88 and 0.93 (p≤0.01), respectively. These relationships indicated that near infrared reflectance analysis had sufficient accuracy to study genotypic variation for total aerial plant protein and changes in protein concentration during ontogeny of spring wheat.

Key Words: Triticum aestivum L. • Kjeldald N • Plant protein

1 Joint contribution from the Minnesota Agric. Exp. Stn. and the USDA, Agric. Res. Serv. Journal Series No. 11,984.

2 Graduate research assistant, and research geneticist and professor, USDA-ARS, Dep. of Agronomy and Plant Genetics, Univ. of Minnesota, St. Paul, MN 55108.

Received for publication November 9, 1981.




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