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Published in Crop Sci 22:1137-1144 (1982)
© 1982 Crop Science Society of America
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In Vitro Production of Haploid Plants of Corn via Anther Culture1

A. D. Genovesi and G. B. Collins2

The objectives of the present study were to confirm the Chinese reports of haploid plant production from corn microspores via anther culture and to further define conditions for improved efficiency. Various genotypes (both Chinese and U.S.A.), media and cold-shock pretreatments of tassels were evaluated. The basal Chinese medium, designated Yu-pei, was modified to test various organic and inorganic additives. Variations in degree and duration of cold pretreatment were studied. Highly significant differences for anthers responding with calli or embryoids were found among genotypes, media, and cold treatments. Two American germplasms, inbred Tx601 and the Armstrong variety, produced plants from cultured anthers. A 14-day cold pretreatment of tassels was found to elicit the best response. Adding activated charcoal to the basal medium further improved response. Under optimum conditions for pretreatment and medium, 18.3% of the treated anters responded. Of those anthers responding with callus or embryoid production, a maximum of 19.2% produced plantlets. Chromosome counts of both haploid and diploid plants were obtained. A total of 20 haploid and diploid plants were grown to maturity and studied in the greenhouse. One haploid/diploid mosaic was selfed while a second pollen plant which could not be selfed was pollenated with pollen from a third anther derived plant.

Key Words: Zea mays L. • Androgenesis • Genotype • Coldshock • Microspores

1 The investigations reported in this paper (81-3-167) were performed in connection with a project of the Kentucky Agric. Exp. Stn. and the paper is published with the approval of the Director.

2 Postdoctoral fellow and professor of agronomy, respectively, Agronomy Dep., Univ. of Kentucky, Lexington, KY 40546-0091. Senior author's current address is Corn Research, DEKALB-Pfizer Genetics, 3100 Sycamore Rd., DeKalb, IL 60115.

Received for publication December 16, 1981.




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