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Evaluation of Vigor Tests in Soybean Seeds: Relationship of Accelerated Aging, Cold, Sand Bench, and Speed of Germination Test to Field Performance¹

Laboratory and greenhouse vigor tests and seed size determinations were performed on 144 and 81 seed lots of soybean [Glycinemax (L.) Merr.] in 1975 and 1976, respectively. These consisted each year of the accelerated aging, cold, and the sand bench test. In 1976, the speed of germination test was included.

Results from these tests and seed weight determinations were compared with field emergence data obtained from these same seed lots at two locations (sandy vs. heavy soil) over three dates of planting each year. The accelerated aging, cold, sand bench, and speed of germination tests were significantly correlated with field emergence in both years, with many of the r values greater than 0.7.

Data from vigor tests presented in this paper and in previous reports were used in computing coefficients of determination and linear regression coefficients for the estimation of final emergence. The coefficients of determination indicated that the estimation of final emergence for a group of seed lots within a particular year may be achieved by using the germination-total live seedlings, from the standard towel, accelerated aging-normal seedlings, accelerated aging-total live seedlings, tetrazolium staining-viable seeds, conductivity/g seed wt., sand bench emergence, cold test emergence and speed of germination-4 day total tests. Linear regression coefficients were not consistent over years for some of the vigor tests. Those vigor tests with the most consistent linear regression coefficients are suggested for use when estimating potential field emergence over years. The vigor tests with the most consistent linear regression coefficients over years were the cold test emergence, tetrazolium staining-viable seeds, accelerated aging-normal seedlings and accelerated aging-total live seedlings. None of the tests are recommended for predicting field emergence.

Key Words: Seed lots • Cultivar • Glycine max (L.) Merr. • Seed quality

² Plant pathologist and plant physiologist, Seed Research Lab., ARS, USDA, Beltsville, MD 20705.

Received for publication April 20, 1981.

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