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Published in Crop Sci 22:716-719 (1982)
© 1982 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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Variation among Inbred Lines of Crimson Clover for N2 Fixation (C2H2) Efficiency1

G. R. Smith, W. E. Knight and H. H. Peterson2

Inbred lines of crimson clover (Trifolium incarnatum L.) were assayed for N2 fixation using the acetylene reduction assay. The seedlings were inoculated with a single pure strain (162K13) of Rhizobium trifolii and cultured in a nil-nitrogen nutrient solution contained in individual plastic growth pouches. The acetylene reduction assay was carried out on live plants using the plastic pouches to contain the gas mixture. In the first experiment, significant differences were noted among 14 crimson clover lines for acetylene reduction. Top dry matter, leaf number, and visual scores of nodulation, root growth and top growth were significantly correlated with the acetylene reduction assay. A relatively large coefficient of variation (27.1%) was noted for this experiment. In the second experiment refinement of culture and assay techniques resulted in a lower coefficient of variation (15.2%) and significant differences among six crimson clover lines for acetylene reduction were found.

Key Words: Acetylene reduction • Rhizobium trifolii • Trifolium incarnatum L.

1 Cooperative investigations of the Mississippi Agric. and Forestry Exp. Stn. and USDA, ARS, Mississippi State, MS 39762.

2 Formerly graduate assistant, Mississippi Agric. and Forestry Exp. Stn. (now assistant professor, Texas A&M Univ., P. O. Drawer E, Overton, TX 75684); research agronomist, Crop Science and Engineering Res. Lab., USDA, ARS; and associate agronomist, Mississippi Agric. and Forestry Exp. Stn., Mississippi State, MS 39762.

Received for publication February 3, 1981.




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