Crop Science Journal of Natural Resources and Life Sciences Education
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Published in Crop Sci 22:428-430 (1982)
© 1982 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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A Scanning Election Microscope Study of Surface Differentiation in Triticale Callus1

Govind C. Sharma and Lateef Bello2

The callus surface of Councill triticale (x Triticosecale Wittmack) was observed using Scanning electron microscopy (SEM) over a 25-day period during proliferation and differentiation. The technique of observation required limited tissue preparation steps. With its ability to scan large surface areas of the tissue, SEM can be utilized in studying stage or sequence of morphogenetc events in callus cultures.

Key Words: Organogenesis • Morphogenesis • In vitro culture • Tissue culture • x Triticosecale Wittmack

1 Research supported by NASA Grant No. NSG 8067.

2 Professor and Research Assistant. Department of Natural Resources and Environmental Studies, Alabama A&M Univ., Normal, AL 35762.

Received for publication February 2, 1981.




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