HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ahloowalia, B. S. Search for Related Content PubMed Articles by Ahloowalia, B. S. Agricola Articles by Ahloowalia, B. S.

Plant Regeneration from Callus Culture in Wheat¹

Plant regeneration was investigated from embryo-callus culture in bread wheat, Triticum aestivurn L. Immature embryos of cultivar ‘Maris Huntsman’ were cultured on half-strength Murashlge and Skoog's medium (half-MS) supplemented with 0.5 mg 2,4-D[(2,4-dichlorophenoxy) acetic acid], 3.2 mg IAA (indole-3-acetic acid) and 1.0 mg kinetin [6-(furfurylamino) purlne]/liter or without kinetin. On medium with 2,4-D, IAA and kinetin, 77% of the embryos produced a visible callus within 2 weeks. On medium without kinetin, all embryos produced a callus. Calll were cloned and maintained for over 900 days by subculturing at 50 to 60 days interval on half-MS medium with 0.5 mg 2,4-D/llter but regeneration ceased after 680 days. Calli showed organogenesis within 120 days of subculture on half-MS medium with 0.5 mg 2,4-D and 1.0 mg zeatin [6-(4-hydroxy-3-methyl but-2 enylamlno) amino purine]/liter. Plantlets originated from callus by differentiation into shoot-primordia and bipolar structures. Plantlets produced roots on half-MS medium without growth regulators; however, 1.0 mg naphthalene acetic acld/llter in the medium promoted rooting. Regenerated plants included variants for plant height, stem thickness, leaf size, spike shape, pollen fertility and seed set.

Key Words: Triticum aestlvum L.. • Embryo culture • Callus induction • Plant regeneration • Embryogenesls • Variants

² Research geneticist

Received for publication March 9, 1981.

This article has been cited by other articles:

				V. V. Satyavathi, P. P. Jauhar, E. M. Elias, and M. B. Rao Effects of Growth Regulators on In Vitro Plant Regeneration in Durum Wheat Crop Sci., September 1, 2004; 44(5): 1839 - 1846. [Abstract] [Full Text] [PDF]