Crop Science Journal of Natural Resources and Life Sciences Education
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Published in Crop Sci 21:778-782 (1981)
© 1981 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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Isoenzyme Polymorphism in St. Augustinegrass1

R. L. Green, A. E. Dudeck, L. C. Hannah and R. L. Smith2

St. Augustinegrasses (Stenotaphrum secundatum Walt. Kuntze) were investigated to determine the feasibility of their identification by isoenzyme variability. Several Florida clones of this species are morphologically indistinguishable especially when grown as turfgrasses under high fertility. Adenosine diphosphate glucose pyrophosphorylase, UDP glucose pyrophosphorylase, alcohol dehydrogenase (ADH), and acid phosphatase isoenzymes extracted from leaf tissue of 28 clones were separated by polyacrylamide disc electrophoresis. Gels of all 28 clones were stained for ADP glucose pyrophosphorylase and UDP glucose pyrophosphorylase isoenzyme activity while gels from selected clones were stained for total protein, ADH, and acid phosphatase isoenzymes. Clones could be divided into several groups by quantitative differences of bands with equal electrophoretic mobility of ADP glucose and UDP glucose pyrophosphorylases. St. Augustine grasses grown in Florida could be divided into two groups by either ADP glucose or UDP glucose pyrophosphorylase isoenzyme systems or total protein. These groups could be further subdivided by acid phosphatase and ADH isoenzymes.

Key Words: Turfgrass identification • Electrophoresis • ADP glucose physophosphorylase • UDP glucose physophosphorylase • Alcohol dehydrogenase • Acid phosphatase • Stenotaphrum secundatum

1 Contribution from Dep. of Ornamental Horticulture, IFAS, Univ. of Florida, Gainesville, FL 32611. Published with the approval of the Director of the Florida Agric. Exp. Stn. as Journal Series Paper No. 2479.

2 Graduate research assistant and associate professor of ornamental horticulture; associate professor of vegetable crops; and professor of agronomy. Institute of Food and Agricultural Sciences, Univ. of Florida, Gainesville, FL 32611.

Received for publication July 16, 1980.




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