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Published in Crop Sci 21:696-699 (1981)
© 1981 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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Tests for a Diffusable Compound Endowing rj1-incompatible Strains of Rhizobium japonicum with the Ability to Nodulate the rj1rj1 Soybean Genotype1

T. E. Devine, B. H. Breithaupt and L. D. Kuykendall2

The nodulation restrictive genotype of soybeans [Glycine max (L.) Merr.] rf1 rf1 is nodulated by some strains of Rhizobium japonicum. Inoculation with mixed cultures of rj1-compatible and rj1-incompatible strains has shown that the presence of an rj1-compatible strain facilitates nodulation of rj1rf1 plants by an rf1-incompatible strain. This study was undertaken to determine whether rj1-compatible strains of R. japonicum produce a diffusable product capable of endowing rf1-incompatible strains with the ability to nodulate the rj1rj1 genotype. An rj1-incompatible strain remained unchanged in ability to nodulate rj1rj1 plants when: (a) grown in culture filtrates of rf1-compatible strains, (b) cultured in U-tubes across a 0.08 nga pore size polycarbonate membrane from rf1-compatible strains, or (c) cultured in dialysis tubing suspended in broth cultures of an rf1-compatible strain. It was concluded that the rf1-compatible strains tested apparently do not produce a diffusable compound in vitro capable of endowing rf1-incompatible strains with the ability to nodulate the rf1rf1 genotype. The rf1-compatible Rhizobium appear to exert their effect, in close proximity to the nodulation site.

Key Words: Glycine max (L.) Merr. • Symbiosis • Root nodule • Microsymbiont

1 Contribution from the Cell Culture and Nitrogen Fixation Lab., Plant Physiol. Inst., USDA, SEA-AR, Beltsville Agric. Res. Ctr. West, Beltsville, MD 20705.

2 Research geneticist, plant physiologist (support scientist) and microbiologist, Cell Culture and Nitrogen Fixation Lab., Beltsville, Agric. Res. Ctr. West, Beltsville, MD 20705,

Received for publication October 3, 1980.




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