HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Swanson, E. B. Articles by Tomes, D. T. Search for Related Content PubMed Articles by Swanson, E. B. Articles by Tomes, D. T. Agricola Articles by Swanson, E. B. Articles by Tomes, D. T.

In Vitro Responses of Tolerant and Susceptible Lines of Lotus corniculatus L. to 2,4-D¹

A positive relationship was established between the in vivo and in vitro 2,4-D [(2,4-dichIorophenoxy) acetic acid] response of two lines of birdsfoot trefoil (Lotus corniculatus L.). Callus cultures were initiated from a line selected for 2,4-D tolerance in the field (T-68), and from a susceptible cultivar ‘Leo.’ The response of the two callus lines was compared with 2,4-D levels lower than the maintenance medium (<1 mg/liter), on progressively higher levels (5, 10, 15, and 20 mg/liter), and on high levels (40 mg/liter) throughout seven subcultures. The Leo callus was more responsive to both decreased, and moderately increased levels of 2,4-D. Growth of both callus cultures was severely inhibited at high levels of 2,4-D. In vitro selection for 2,4-D tolerance appears to be practicable in Lotus corniculatus L. Indications are that 2,4-D conjugation is one possible mechanism for differential tolerance in this species.

Key Words: Birdsfoot trefoil • Cell culture

² Research assistant and assistant professor, respectively, of the Dep. of Crop Science, Univ. of Guelph, Guelph, Ontario, NIG 2W1.

Received for publication November 19, 1979.