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Haploid Plants by Anther-Panicle Culture of Tall Fescue¹

Haploid plants were cultured from ‘Kentucky 31’ tall rescue (Festuca arundinacea Schreb.). Field-grown plants were cut at the soil surface when the panicles showed about 3 cm above the flag leaf. The plants were kept in a flask of tap water and preconditioned in darkness at 5 C before cultures were made. Florets attached to about 2.5 cm of panicle tissue were cultured on modified Murashige and Skoog medium containing 2 mg of 2,4-dichlorophenoxyacetic acid (2,4-D)/liter. The panicle segments served as nurse tissue. Loose proliferations of cells emerged from several anthers after about 5 weeks. Some plantlets became apparent at about 7 weeks. More than 30 green plantlets formed. There were no albino plantlets. Examination of metaphase cells in root and shoot tips showed that 22 of the 23 plants examined had the haploid chromosome number, n = 21. The plants grew vigorously after transfer to pots in a plant growth chamber.

Key Words: Festuca arundinacea Schreb. • Plant tissue culture • Monocot haploids • Anther culture • Forage grass genetics

² Plato physiologist, research agronomist, and research geneticist, respectively, USDA, SEA, AR, also professors and assistant professor, Dep. of Agronomy, Univ. of Kentucky, Lexington, KY 40546.

Received for publication May 10, 1979.