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Improved Rate of Callus and Green Plant Production from Rice Anther Culture Following Cold Shock¹

Rice (Oryza sativa L. subsp. japonica) panicles containing pollen at the G₁ G₂, early binucleate, and late binucleate stages of development were subjected to 5, 10, and 13 C temperatures for 3, 7, 10, 14, and 21 days before placing the anthers on callus-inducing medium. The uninucleate stages gave much higher frequencies of callus induction in both control and treated samples. In general, the more moderate temperature over a 10- to 14-day treatment gave maximum frequency of callus induction. While most of the plants derived from G₁ and G₂ stage anthers stored in the cold for 2 weeks or less were green, plants from later pollen stages or 21 days of cold storage were predominately albino.

Key Words: Oryza sativa L. • Tissue culture • Plant organogencies

² Research associate, Agronomy Dep., Univ. of Kentucky, Lexington, KY 40506 and associate professor of genetics, Texas A&M Univ., College Station, TX 77843.

Received for publication February 20, 1979.