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Regeneration of Plantlets from Callus Cultures of Indiangrass¹

Plantlets were regenerated through organogenesis from the callus cultures of 10 agronomically superior genotypes of Indiangrass (Sorghastrum nutans L Nash). Callus was first initiated from segments of young inflorescences explanted on RM medium supplemented with hormonal combinations of 5 or 10 mg 2,4-D and 0 or 0.2 mg kinetin/liter. Plantlets were then induced from the calluses by subculturing on RM medium with kinetin omitted and 2,4-D reduced below 1 mg/liter. Of the media tested, callus grew best on RM medium supplemented with 5 mg 2,4-D and 0.2 mg kinetin/liter. Differential responses to the morphogenetic induction medium were noted among the 10 genotypes tested.

Key Words: Sorghastrum nutans L. Nash • Tissue culture • Organogenesis • 2,4-Dichlorophenoxyacetic acid • Kinetin • Plant breeding

² Professor and graduate research assistant, Botany-Biology Dep. and professor of Plant Science, respectively, South Dakota State Univ., Brookings, SD 57007.

Received for publication June 5, 1978.