Crop Science Journal of Natural Resources and Life Sciences Education
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Published in Crop Sci 18:955-958 (1978)
© 1978 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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Isozyme Patterns and Differences in Plant Parts and Their Callus Cultures in Common Bean1

A. Bassiri and P. S. Carlson2

Changes that occur in different enzyme systems during the culture of cells or callus have been extensively studied in several plant species. A question exists whether different tissues of the same plant maintain persistent tissuespecific patterns of gene expression during in vitro culture or whether in vitro cultures are characterized by identical patterns of gene expression no matter what their tissue of origin. This question is of importance to crop improvement since many genetic selections in vitro and rapid assays for agronomic traits in vitro require the cellular expression of tissue specific traits.

Extracts of primary leaf, epicotyl tip, epicotyl, cotyledon, mesocotyl, and radicle tissues of Phaseolus vulgaris L. and of callus derived from them were subjected to electrophoretic separation and assays of cathodal and anodal peroxidases and malate dehydrogenases and anodal acid phosphatase isozymes. The patterns of most of the isozyme systems were specific at the organ tissue level; however, a great uniformity of isozyme pattern occurred within and between the calli of the same or different origins. Rapidly growing calll derived fxom different plant parts expressed similar, though not in all cases identical, isozyme patterns.

Key Words: Starch gel electrophoresis • Peroxidases • Malate dehydrogenases • Phosphatase • Phaseolus vulgaris

1 Contribution from the Dep. of Agronomy, Pahlavi Univ., Shiraz, Iran and Dep. of Crop and Soil Sciences, Michigan State Univ., East Lansing, MI 48824, and supported in part by CSRS Research Contract No. 616-15-61 of MSU. The senior author gratefully acknowledges a Fulbright-Hays Fellowship.

2 Associate Professor and Professor, respectively.

Received for publication January 3, 1978.




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