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The inheritance of a male-sterile condition in red clover (Trifolium pratense L.) was determined. Appropriate crosses were made onto a male-sterile clone, 740, and onto clone 66 which possessed a previously identified malesterile gene (ms1). Segregations for male-fertility and male-sterility were observed in the following generations: F1, generation, F1 and F2 testcrossed to respective parent clones and F1's, F2 generation from sib-mated F1's and sib-mated F2's. All ratios observed indicated a second single recessive gene was responsible for male-sterility in clone 740. This locus was apparently independent from ins, of clone 66 and also from the incompatibility factors (S-alleles). Accordingly, this locus was designated ms2 to distinguish it from ms1. The two loci (ms1 and ms2) interacted in the sib-mated F2 generation to give a duplicate recessive epistatic type of gene action as indicated by a 9:7 ratio.
Key Words: Duplicate recessive epistasis Double-cross hybrids Trifolium pratense L.
2 Professor of agronomy, graduate assistant, and associate professor of agronomy (now senior barley breeder, North American Plant Breeders, Berthoud, CO 80513), Univ. of Kentucky, Lexington, KY 40506, respectively.
Received for publication May 9, 1978.
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