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Effects of Sucrose Concentration on Plant Production in Anther Culture of Rice¹

Rice (Oryza sativa L.) anthers of various developmental stages were cultured on medium with 3%, 6%, and 9% sucrose to test the effects of sucrose concentration on plant production. It was found that high sucrose concentrations in the callus-induction medium promoted callus formation and subsequent organogenesis. However, callus initiated on the 9% sucrose medium differentiated more albino plants than callus from the medium with lower sucrose concentrations.

Key Words: Oryza sativa L. • Pollen callus • Plant regeneration

² Professor, Dep. of Botany, National Taiwan Univ., Taipei, Taiwan, Republic of China.

Received for publication May 12, 1978.