Crop Science Journal of Natural Resources and Life Sciences Education
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Published in Crop Sci 18:489-490 (1978)
© 1978 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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A Colorimetric Screening Assay for Lysine in Wheat Using Trinitrobenzesulfonic Acid1

Peter Felker, C. K. Labanauskas and Giles Waines2

A colorimetric lysine screening assay in wheat is reported that employs trinitrobenzenesulfonic acid, which reacts only with primary amino proups. When lysine in 26 wheat samples is compared with that determined by traditional amino acid analysis, the assay has a correlation coefficient of 0.938. It is possible to perform 48 lysine determinations per man-day.

Key Words: Triticum monococcum • Triticum aestivum

1 Contribution from the Dep. of Plant Sciences, Univ. of California, Riverside, 92521.

2 Postgraduate research plant physiologist, professor, and assistant professor, Univ. of California, Riverside.

Received for publication September 2, 1977.




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