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Published in Crop Sci 18:210-216 (1978)
© 1978 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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Fine Structure of Sugarcane (Saccharum sp.) Callus and the Role of Auxin in Embryogenesis1

H. M. Nadar, S. Soepraptopo, D. J. Heinz and S. L. Ladd2

Histological examination of callus of sugarcane cultivar H50-7209 (Saccharum sp. hybrid) revealed all stages of embryoid development when the callus was cultured on modified Murashige and Skoog medium containing 3.0 mg 2,4-D/liter. It is suggested that the process of dedifferentiation and embryogenic-cell initiation required relatively high auxin concentrations. Lower auxin concentrations may be required for advanced embryogenesis stages. Plantlet differentiation occurred only in the absence of auxin. Callus on auxin-free medium showed no dedifferentiation or embryogenesis. We suspect that the origin of the individual sugarcaue plants in this study is from single cells. If so, mutant induction and selection could yield true mutant plants.

Key Words: Differentiation • Dedifferentiation • Embryogenic cell • Embryoid development

1 Contribution from Experiment Station, Hawaiian Sugar Planters’ Association, Aiea, Hawaii 96701. Research supported in part by funds provided by U.S. Department of Agriculture, Agricultural Research Service Cooperative Agrecmeut No. 12-14-5001-83. Published with the approval of the Director as Journal Series Paper No. 419, Experiment Station, Hawaiian Sugar Planters’ Association.

2 Formerly assistant plant breeder, present address: U.S. A.I.D., P.O. Box 30261, Nairobi, Kenya; IAEA/NRC research fellow, present address: Yogyakarta Plantation Institute (L.P.P.) Jl Solo 40A, Box 6, Yogyakarta Indonesia; head, Dep. of Genetics and Pathology; formerly plant breeder, present address: Associate Professor, Dep. of Agronomy, Colorado State Univ., Fort Collins, CO 80523.

Received for publication October 14, 1976.




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