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Published in Crop Sci 17:963-965 (1977)
© 1977 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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Variety-Specific Electrophoretic Variants of Four Soybean Enzymes1

M. B. Gorman and Y. T. Kiang2

The purpose of this study was to examine the possible use of electrophoresis in soybean (Glycine max (L.) Merr.) variety identification. Seeds from 113 commercial soybean varieties were examined with polyacrylamide slab gel electrophoresis. Distinct variety-specific electrophoretic zymogams were observed for acid phosphatase (AP), alcohol dehydrogenase (ADH), amylase (AM), tetrazolium oxidase (TO). The varieties displayed one of three banding patterns (zymograms) for each of the four enzymes. In the 4,411 seeds tested for these four enzymes, only four seeds yielded zymograms which were atypical of their respective variety. An additional nine seeds were probably of heterozygous genotype based upon the observed zymograms. Thus, the electrophoretic zymograms were stable varietal characteristics and could be used in varietal identification. Varietal pedigrees constructed from the available records indicated that variant dectrophoretic zymogram patterns were heritable, and that all variants could be traced back to an area of origin.

Key Words: Polyacrylamide gel electrophoresis • Enzyme variants • Soybean varietal identification

1 Published with the approval of the Director of the New Hampshire Agric. Exp. Stn. as Scientific Contribution No. 861.

2 Graduate research assistant, presently Dep. of Agronomy, Iowa State Univ., Ames, and associate professor of plant science and genetics, Univ. of New Hampshire, Durham, NH 03824, respectively.

Received for publication April 11, 1977.




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