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Preliminary Study of Pachytene Morphology in a Homozygous Line of Sugarbeet¹

The morphology of the pachytene chromosomes of a homozygous Beta vulgaris L. line 6600 (2n = 18) was analysed. Observations were made on squash preparations of alcoholic hydrochloric acid-carmine stained microsporocytes. Interpretation of the sugarbeet pachytene chromosomes was difficult because they failed to spread; nonetheless, the bivalents were distinguishable by the patterns of chromomere distribution. The physical lengths of the nine chromosomes were measured and found to range from 12.4 to 33.7 microns, with a total length of 214.3 microns. Based on the probable centromere positions observed, the complement consisted of three chromosomes with median centromeres and six with submedian. The pattern of chromomere distribution of this species was nonproximal. Information on the pachytene morphology will facilitate cytogenetic analysis of structural variation in the chromosome complement.

Key Words: Beta vulgaris L. • Pachytene analysis • Cytogenetics • Idiogram

² Research agronomist, Agric. Res. Set., USDA, P. O. Box 5098, Salinas, CA 93915.

Received for publication February 25, 1977.