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Published in Crop Sci 17:814-816 (1977)
© 1977 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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Root and Shoot Development from Sugarcane Callus Tissue1

H. M. Nadar and D. J. Heinz2

Growth of shoots differentiated from sugarcane (Saccharum sp.) callus was enhanced by 5 mg naphthalene acetic acid (NAA)/I in the absence of coconut water, while 0.2 mg 2,4-dichlorophenoxyacetic acid (2,4-D)/1 stunted their growth. In the presence of NAA, a crown developed at the base of the shoot on which new shoots and roots developed, grew, and formed a clump of plantlets.

Root growth could also be induced by trimming the leaves or by incubation at 15C for 4 to 6 weeks. Leaf trimming resulted in more vigorous root growth than cold treatment, although clonal differences were observed.

Results indicate that a medium which stimulates differentiation does not necessarily stimulate shoot growth, and the hormonal requirements for shoot differentiation differ from requirements for root induction in sugarcane callus.

Key Words: Root induction • Nutrient medium • Stool formation • NAA • 2,4-D

1 Contribution from Dep. of Genetics and Pathology, Hawaiian Sugar Planters' Association Exp. Stn., Aiea, Hawaii. Research supported in part with funds provided by the USDA, ARS Cooperative Agreement No. 12-14-5001-83. Published with the approval of the Director as Paper No. 411 in the Journal Series of the Experiment Station, Hawaiian Sugar Planters' Association.

2 Formerly assistant plant breeder. Present address: U.S.A.I.D., P.O. Box 9130, Dar es Salaam, Tanzania; and head, Dep. of Genetics and Pathology.

Received for publication December 9, 1976.




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