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Relationship of Ribonucleic Acid Degrading Enzymes to Survival Levels of Winter Barley Phenotypes¹

The ribonuclease and phosphodiesterase enzyme activities of 18 lines of barley (Hordeum vulgare L.) were compared to their winter survival ratings. These lines, selected in Wyoming, were obtained from a composite cross derived from 18 original parents using male sterility. Enzyme analysis on the soluble extract from 4-day seedlings showed, in general, that the rihonuclease activity in the hardiest phenotypes was depressed to a greater extent by 0.50 M KCI. These extracts contained the lowest amounts of an enzyme tentatively identified as ribonuclease L Usually the extracts of the hardiest phenotypes also contained the highest phosphodiesterase activity, as determined with thymidine 3'-(p-nitrophenyl) phosphate.

Key Words: Hordeum • Winter survival • Ribonuclease • Phosphodiesterase • Electrophoresis

² Professors of plant science aod chemistry, South Dakota State Univ., respectively.

³ Professor of Plant Science, Univ. of Wyoming.

Received for publication March 3, 1976.