Crop Science Grow Your Career with CSSA
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Published in Crop Sci 17:445-448 (1977)
© 1977 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Amos, J. A.
Right arrow Articles by Scholl, R. L.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Amos, J. A.
Right arrow Articles by Scholl, R. L.
Agricola
Right arrow Articles by Amos, J. A.
Right arrow Articles by Scholl, R. L.

Effect of Growth Temperature on Leaf Nitrate Reductase, Glutamine Synthetase and NADH-Glutamate Dehydrogenase of Juvenile Maize Genotypes1

J. A. Amos and R. L. Scholl2

Ten F1 hybrids of maize (Zea mays L.) grown at two day/night temperatures (35/25 C and 40/28 C) in controlled-environment chambers were assayed for in vitro nitrate reductase (NR) to compare the relative effect of high temperature on nitrate reductase activity (NRA) among hybrids. High temperature response of nitrate reductase was tested by assaying glutamine synthetase (GS) and NADH-glutamate dehydrogenase (GDH) from temperature-treated seedlings in a set of genotypes that differed for the degree of high temperature stability of nitrate reductase. The NRA of most hybrids was reduced approximately 35% by high temperature, and the overall genetic variability of NRA for temperature stability was low. However, the NRA of some hybrids, such as ‘A632Ht X B37Ht’, was depleted less by high temperature (22 and 17% reduction in two experiments, respectively) than others, such as ‘Oh51A X Oh551X (54 and 31% reduction), indicating that genotypic differences can occasionally be large. Genotypic variability for NR response to temperature was not correlated with that for GS or GDH, neither of which activities were reduced significantly by high temperature in any of six genotypes. The effect on NR of the addition of protein to the NR extraction medium was tested using tissue from seedlings grown at high and low temperatures. This addition had no detectable influence on the apparent temperature stability of genotypes. Protein addition, however, did slightly increase NRA of some of the genotypes. Increases of NRA due to addition of bovine serum albumin ranged from 0 to 30%

Key Words: Heterosis • Zea mays L. • Nitrate reductase extraction procedures

1 Contribution from the Dep. of Genetics, The Ohio State Univ., Columbus, OH 43210. Portions of this contribution were adapted from an M.S. thesis by the senior author.

2 University fellow and assistant professor of genetics, respectively.

Received for publication May 11, 1976.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
The SCI Journals Agronomy Journal Vadose Zone Journal
Journal of Natural Resources
and Life Sciences Education		 Soil Science Society of America Journal
Journal of Plant Registrations Journal of
Environmental Quality		 The Plant Genome
Copyright © 1977 by the Crop Science Society of America.