HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Wehner, D. J. Articles by Watschke, T. L. Search for Related Content PubMed Articles by Wehner, D. J. Articles by Watschke, T. L. Agricola Articles by Wehner, D. J. Articles by Watschke, T. L.

Separation of Kentucky Bluegrass Cultivars using Peroxidase Isoenzyme Banding Patterns¹

The peroxidase isoenzyme banding pattern of 15 Kentucky bluegrass (Poa pratensis L.) cultivars was investigated for use in identification. Isoenzymes were separated by polyacrylamide slab gel electrophoresis, stained with benzidine-H₂0₂, and the relative intensities of the bands evaluated with a densitometer. Cultivar separations were made on single seedling samples at the 1% level of confidence using band number, mobility, and intensity ratios. Overall variability of the procedure was evaluated using a horeradish peroxidase standard and the effects of seedling age and seedlot determined. Of the 15 cultivars, 11 could be separated individually, whereas the remaining 4 were grouped into two pairs. Band mobilities for the standard and the cultivars varied 1 to 3%, whereas intensity ratios varied 9% for the standard, and 5 to 20% for most cultivars. Neither seedlot, seedling age, nor leaf position on the same seedling affected the peroxidase isoenzyme banding pattern.

Key Words: Poa pratensis L. • Electrophoresis

² Graduate assistant, professor, and associate professor, respectively. The Pennsylvania State Univ., University Park, Pa.

Received for publication November 12, 1975.

This article has been cited by other articles:

				D. W. Lickfeldt, T. B. Voigt, and A. M. Hamblin Cultivar Composition and Spatial Patterns in Kentucky Bluegrass Blends Crop Sci., May 1, 2002; 42(3): 842 - 847. [Abstract] [Full Text] [PDF]