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Laboratory Technique for Bleaching Pea Seeds¹

Conditions for bleaching pea (Pisum sativum L.) seeds in the laboratory were studied to find a method of identifying bleach-resistant selections in the early stages of a breeding program. Among the genotypes used, differential seed bleaching without germination was obtained when 4.2 g pea seed (about 25 seeds) was placed on five sheets of Whatman no. 1 filter paper in 9-cm petri dishes and moistened with 6 ml water. The petri dishes were then placed in a growth chamber at 23 C with 8.6 klux of continuous light. After 8 days, the seed coats were removed, and the cotyledons were scored on a scale from 0 for no bleaching to 4 for complete bleaching. The technique was highly correlated (r = 0.78**, df = 27) with seed bleaching occurring in the field and should therefore be useful for early generation testing of green, dry-edible pea selections.

Key Words: Cotyledon color • Pisum sativum L.

² Former graduate assistant, Dep. of Agronomy and Soils, Washington State Univ.; and research geneticist, ARS-USDA, Dep. of Agronomy and Soils, Washington State Univ., Pullman, WA 99163.

Received for publication May 22, 1975.