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Tissue Culture of a Tobacco Albino Mutant¹

This report describes the survival and successful in vitro culture of albino tobacco tissues normally lethal during seedling development. The culture of the lethal homozygous tissue requires a complete tissue culture medium supplemented with auxins of the indole-3-acetic acid type. In vitro the tissue of the albino phenotype grows as rapidly under low light or in the dark as tissues of the normal green type.

Key Words: Indole-3-acetic acid • Lethal • Yellow mutant (Su/su)

² Plant physiologists, ARS, USDA, Beltsville Md., and Morgantown, W. Va., respectively.

Received for publication January 27, 1975.