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Physiological Significance of the In Vivo Assay for Nitrate Reductase in Cotton Seedlings¹

In vivo assay techniques for nitrate reductase activity were evaluated for their ability to predict nitrate assimilation rates of germinating dark-grown cotton (Gossypium hirsutum L.) seedlings. Activity was measured with four in vivo assay techniques: either aerobic or anaerobic, and either with or without added nitrate in the assay medium. Assays without added nitrate in the medium allowed closer estimates of reduced N accumulation than did assays with added nitrate. Additionally, in the absence of added nitrate, the anaerobic assay provided a better estimate than the aerobic assay because of O₂-stimulated nitrite reduction. The results suggest that the in vivo assay for nitrate reductase best approximated nitrate assimilation rates with only endogenous nitrate as substrate.

Key Words: Gossypium hirsutum L. • Microdiffusion analysis • Nitrate ion electrode

² Plant physiologist and physical sciences technician, ARS, USDA, Western Cotton Res. Lab., 4135 E. Broadway Rd., Phoenix, AZ 85040, and associate professor, Dep. of Plant Sci., Tex. A&M Univ., College Station, TX 77843, respectively.

Received for publication February 18, 1975.

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